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Fluorescent imaging

Fluorescent imaging Platform

Equipment Type IVIS Lumin Imaging System
Capabilities Small animal imaging in vivo
Status Active
Reserve time 9:00--18:00
Contact staff Qingliang  Zhao
Application form PDF icon PDF
Email xiamen-cmitm@xmu.edu.cn

Performance
    The IVIS Lumin is the most versatile and advanced in vivo small animal imaging system in biofluorescence imaging field.
The two photon excitation microscopy has become an important tool of noninvasive imaging due to the better penetration and relative harmlessness of the longer wavelength.
    An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas.
    For advanced fluorescence imaging, the IVIS Lumin has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources.

  • 3D diffuse fluorescence tomography
  • 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth)
  •  Multiple fluorescent reporters within the same animal

Application

 

Fluorescent Imaging (Carestream FX Pro) Platform

Equipment Type Carestream FX Rro system
Capabilities Small animal imaging
Status Active
Reserve time 9:00--18:00
Contact staff Qingliang  Zhao
Application form PDF icon PDF
Email xiamen-cmitm@xmu.edu.cn

Performance

The In-Vivo Multispectral FX PRO features automated image capture including powerful excitationbased spectral unmixing. The resulting enhanced sensitivity enables identification and separation of multiple fluorophores and removal of autofluorescence background. The MS FX PRO automatically generates multispectral image “cubes” with spatially co-registered X-ray and white light images for improved localization of biomarkers in vivo. This co-registration also enables direct correlation of molecular signals to phenotypes that change bone structure, bone density or animal appearance.

Powerful Multispectral Analysis:

◆ Powerful software models data and provides unmixing of fluorophores

◆ Easily establish, store and repeat multi-modal longitudinal imaging protocols

◆ A wide range of excitation wavelengths enables optimum imaging of a wide range of fluorophores and biomarkers

◆ Sophisticated software algorithms remove autofluorescence for improved signal-to-noise and detection sensitivity.

◆ Unique imaging geometry improves image clarity by providing a true flat focal plane

◆ Multimodal imaging capabilities include multi-wavelenth fluorescence, white light, luminescence, radioisotopic and X-ray

◆ Automated excitation and emission filter systems with 29 excitation filter positions and 4 emission filter positions

◆ Large 20 cm x 20 cm field of view for imaging multiple subjects simultaneously using all modalities

◆ Automated imaging chamber allows remote switching between imaging modes without disturbing the subject

Application

 

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