Mitochondria-Targeting, Intracellular Delivery of NativeProteins Using Biodegradable Silica Nanoparticles

Angewandte Chemie 17 April 2019  DOI:10.1002/ange.201901699Bionanotechnolo

Peiyan Yuan, Xin Mao,Xiaofeng Wu,SiSiLiew,Lin Li,and Shao Q. Ya

Mitochondriaarekeyorganellesinmammaliancells whose dysfunction is linked to various diseases.Drugs targeting mitochondrial proteins provideahighly promising strategy for potential therapeutics.Methods for the delivery of smallmolecule drugs to the mitochondria are available,but these are not suitable for macromolecules,such as proteins.Herein, we report the delivery of native proteins and antibodies to the mitochondria using biodegradable silica nanoparticles (BS– NPs). The modification of the nanoparticle surface with triphenylphosphonium (TPP) and cell-penetrating poly(disulfide)s (CPD) facilitated their rapid intracellular uptake with minimal endolysosomal trapping,providing sufficient time for effective mitochondrial localization followed by glutathionetriggeredbiodegradationandofnative,functionalproteinsinto the mitochondria

原文链接：https://onlinelibrary.wiley.com/doi/full/10.1002/ange.201901699