Tamara Maric, Georgy Mikhaylov, Pavlo Khodakivskyi, Arkadiy Bazhin, Riccardo Sinisi, Nicolas Bonhoure, Aleksey Yevtodiyenko, Anthony Jones, Vishaka Muhunthan, Gihad Abdelhady, David Shackelford & Elena Goun

Nature Methods 13 May 2019 DOI:10.1038/s41592-019-0421-z

Abstract

Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used ¹⁸F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development.

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  原文链接 https://www.nature.com/articles/s41592-019-0421-z